Bioluminescence Bioluminescence is the production and emission of light by a living organism as the result of a chemical reaction. In this process chemical energy is converted into light energy. Bioluminescence can be generated by the organism itself or by symbiotic organisms carried within larger cells (Science Reference Bioluminescence). It is generated by an enzyme-catalyzed chemoluminescence reaction‚ where a luciferin is oxidized by a luciferase‚ and in most cases an ATP is involved. This
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Bioluminescence Have you ever experienced a light show underwater? No‚ you are right that is not and can not be actually lights with electricity‚ but is the notorious scientific discovery of bioluminescence. One of the earliest sightings was by Aniximenes in 500 B.C. where he described that the alluring light show as “glowing when he struck the sea with an oar”. People have observed bioluminescence countless times ever since. In the Mesopelagic zone of the ocean‚ which is about 200m to 1000m
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Bioluminescence in Fungi INTRODUCTION What is Bioluminescence? The current paper main focus is on bioluminescent Fungi but the basic features of bioluminescence discussed are common to all bioluminescent organisms. Bioluminescence is simply light created by living organisms. Probably the most commonly known example of bioluminescence by North Americans is the firefly‚ which lights its abdomen during its mating season to communicate with potential mates. This bioluminescent ability occurs in
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the recombinant plasmids and the competent cells through heat shock transformation. 5. The transformation was then plated on LB/amp/X-gal plates. 6. The plates were screened for our target with ampicillin resistance‚ blue/white colony‚ then bioluminescence. 7. A bioluminescent was selected and re-streaked. 8. A non-glowing white colony was selected sent out for sequencing. Ligation a. Tube Insert to Vector Ratio Digested V. fischeri (insert) DNA Digested pGEM H2O 5X Ligation Buffer
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Bioluminescence is typically associated with dinoflagellates as a form of a chemical reaction that happens within those organisms and the product is light. Bioluminescence Imaging is similar in that it is a byproduct of light emission‚ however it has a greater importance than just giving off light or being a relatively interesting chemical reaction. Bioluminescence Imaging is a technology that has been developed recently in the last few years and allows for real-time analysis of diseases at the molecular
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BIOSENSORS ADVANCED BIOREPORTER TECHNOLOGIES FOR TARGETED SENSING OF CHEMICAL AND BIOLOGICAL AGENTS Steven Ripp and Gary S. Sayler Introduction Bioreporters refer to intact‚ living microbial cells that have been genetically engineered to produce a measurable signal in response to a specific chemical or physical agent in their environment (Figure 1). Bioreporters contain two essential genetic elements‚ a promoter gene and a reporter gene. The promoter gene is turned on (transcribed) when the
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are used to highlight samples of blood at a crime scene. The two chemicals react readily due to the iron present in haemoglobin‚ giving off a luminescent glow as a result. Chemiluminescence occurs even in living organisms where it is termed bioluminescence. Using fireflies as an example‚ a reaction where luciferin combines with adenosine tri-phosphate (ATP) and reacts with the enzyme luciferase. The action of the enzyme acting on luciferase simulates something similar to luminol and a peroxide being
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the variables to this particular lab are as such: that Plate A (no plasmid or ampicillin) will show colonies but no bioluminescence‚ Plate B (Plasmid DNA but no ampicillin) will show colonies and bioluminescence‚ Plate C (no plasmid but ampicillin present) will show no colonies and no bioluminescence‚ and Plate D (Plasmid DNA and ampicillin present) will show colonies and bioluminescence. For the variables in this lab are two‚ first would be independent and second dependent. The independent variables
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cells will be different. I predict that the E. coli cells containing no ampicillin will be able to grow colonies. I also predict that the plates with plasmid DNA will show signs of bioluminescence. The plate with ampicillin present with no plasmid DNA will not be able to grow colonies and will not be capable of bioluminescence. Methods: The following experiment method is based on the procedure given through the Biology Department at UWM (Wimpee‚ 2006). This experiments started with two tubes of 100
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ultimate goal of this experiment is to isolate the lux operon‚ a targeted piece of DNA that causes bioluminescence‚ from Aliivibrio fischeri and insert it into the DNA of Escherichia coli in order to make it glow. A. fischeri is a gram-negative bacteria which participates in a symbiotic relationship with many marine organisms (Perry et al.‚ 2005). This particular bacterium has the feature of bioluminescence‚ which is regulated by a ~nine-kilobase fragment of their chromosomal DNA‚ the lux operon (Slock
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