Protocol for Lab 5 – Aerobic Respiration Part 1 Isolation of Mitochondria from Cauliflower - Weigh 50g of rosettes cut from fresh cauliflower head. - Cut rosettes and place it on ice - Prepare juice extractor by placing ice and an empty 150 ml beaker into the right compartment. - Collect pulp from left compartment and record total volume of the extract. Approx. 20ml - Filter the pulp using six layered cheese cloth and collect it in a beaker sitting on ice. - Place two 50 ml test tubes
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Toxicology Lab 1. In this investigation‚ a wide range of concentrations of Sodium Chloride (NaCl) solution were created and the effects that they had on radish seeds were tested. This ultimately created a doseresponse experiment in which it was detectable whether or not radish seeds were a reliable bioassay for the toxicity of NaCl. The goal of this experiment was to determine a correlation between toxicity and seed germination/radicle
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The Virtual Lab – ELISA Test Lab: Immunology 09/04/2013 Instructors: Dr. Charlie Wilson Written by: Dipen Patel I. Objective: The purpose of the lab was to learn the procedure of performing an ELISA test to determine whether a particular antibody is present in a patient’s blood sample. ELISA is an abbreviation for “Enzyme-linked Immunosorbent Assay." II. Introduction: The interaction of antigen and antibody outside the body can be used to determine if patient
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reaction. Name one microbe that would produce this response. * This change to the media will impart a blue color throughout the media or cause a blue band to form at atop of the media. This PH change is a result of proteolytic and degradative enzymes producing ammonia or amines form the breakdown of proteins. * Proteus
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Biology Lab report Proteins are very important throughout the body. They have a lot of functions in cells. Antibodies are proteins that help cells rebuild the cells when they are destroyed. Transport proteins move substances from one place to another. Regulatory proteins control cellular metabolism in a way that cells throughout the body can have the right amount of glucose in the blood. A common protein is an enzyme; in cells they speed up chemical reactions. For an example‚ “amylase is an enzyme
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carbohydrates begin in the mouth‚ where an enzyme‚ salivary amylase (α-amylase;ptyalin) starts to breaking the polysaccharides (starch) into short polysaccharides (dextrin). Dextrin is a partial degradation of starch‚ shorter chains of maltose units. Salivary amylase is inactivated by stomach acid in the stomach and to a small extent‚ it continues breaking down starch (but there’s no enzymatic activities on carbohydrates in the stomach). An intestinal enzyme‚ pancreatic amylase‚ continues the activity
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Studying the Rate of Reaction of catechol oxidase and how it affects pH levels Introduction: In this lab‚ we studied the activity of an enzyme that is found in fruits and vegetables called catecholase or catechol oxidase. An enzyme is a protein molecule that is a catalyst. Catechol oxidase is the enzyme in fruits and vegetables that turns them that undesirable brownish color; also commonly referred to as bruising or bruised. When walking through your regular grocery store and you find yourself in
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of the increase in the enzyme concentration on the rate of reaction. By using self investigative and experimental skills‚ the experiment was done in order to determine how the rate of reaction will be altered‚ whether it will increase‚ decrease or remain constant when the different concentration of enzymes added. INTRODUCTION: Enzymes are produced naturally in plant‚ animal‚ and microbial cell. There are thousands of different enzyme can be found in any cell. Enzymes can be describe as molecules
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Lab 5 The Diffraction Grating Chinua McDonald Objective: To measure the wavelength of light with a diffraction grating. Theory: The two types of diffraction gratings are the transmission and reflection gratings. They are made by ruling on a piece of glass or metal a number of evenly spaced lines with a fine diamond point. Diffraction phenomena can be analyzed in terms of Huygens’ principle‚ according to which every point on the wave front of a wave should be considered as a source
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Hydrolysis of starch for fungal amylase Aspergillus Oryzae and bacterial amylase Bacillus Licheniformis at different temperatures. Maydelis Perez PI 4593042 Florida International University BSC 1010L‚ section UO9‚ March 6‚ 2013 Abstract Enzymes are very specific protein because they contain one active site on their surface that enable the substrate to bind to the enzyme and form the enzyme substrate complex and then release
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